The patent has been granted The patent has been granted, Grant of patent is intended, Examination is in progress, Request for examination was made EP Register Verified

A METHOD FOR PRODUCING AND PURIFYING RNA, COMPRISING AT LEAST ONE STEP OF TANGENTIAL FLOW FILTRATION

Publication No. EP4108769

•

App No. EP22190069

•

Filed by CureVac Manufacturing GmbH

•

Filed on 2016-05-30

•

Issued/Published on 2023-08-30

Official EPO Register

Publication No. EP4108769 A1

Application No. EP22190069 Filed: 2016-05-30

Grant Date 2016-05-30 Mention of grant

Active Oppositions 6 Active EPO Oppositions

Patent Abstract / Summary

[0001] The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

IPC Classifications

C12N15/10, C12P19/34

Priority Claims

WO 2015EP62002 Date: 2015-05-29

Designated Contracting States (38 EPC States)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

Parties & Representation

Applicants

CureVac Manufacturing GmbH

Friedrich-Miescher-Straße 15, 72076 Tübingen

CureVac Real Estate GmbH

Friedrich-Miescher-Straße 15, 72076 Tübingen

Inventors (7)

FUNKNER, Andreas

72076 Tübingen

DORNER, Stefanie

72076 Tübingen

SEWING, Stefanie

72076 Tübingen

KAMM, Johannes

72076 Tübingen

BROGHAMMER, Norbert

72076 Tübingen

KETTERER, Thomas

72076 Tübingen

MUTZKE, Thorsten

72076 Tübingen

Representatives / Agents

Pinsent Masons LLP

30 Crown Place, London EC2A 4ES

Maiwald GmbH

Elisenhof, Elisenstraße 3

Cohausz & Florack

Patent- & Rechtsanwälte, Partnerschaftsgesellschaft mbB

Pfizer

European Patent Department, 23-25 avenue du Docteur Lannelongue

Carpmaels & Ransford LLP

One Southampton Row, London WC1B 5HA

Hoffmann Eitle

Patent- und Rechtsanwälte PartmbB, Arabellastraße 30

Key Procedural Dates

Request-For-Examination 2022-08-11

Mention Of Grant Published 2016-05-30

First-Examination-Report-Despatched 2023-03-07

Patent Oppositions

Official opposition proceedings filed against European Patent EP4108769 under EPC Article 99.

6 ACTIVE OPPONENTS

Opponent Company

COHAUSZ & FLORACK Patent- und Rechtsanwälte Partnerschaftsgesellschaft mbB

Bleichstrasse 14, DE

Representative

Cohausz & Florack

Filing Date

2024-02-27

Opposition in Progress

Opponent Company

Höpfner, Sebastian

c/o ZSP Patentanwälte, DE

Representative

—

Filing Date

2024-05-29

Opposition in Progress

Opponent Company

Pfizer Inc.

66 Hudson Boulevard East, US

Representative

Pfizer

Filing Date

2024-02-29

Opposition in Progress

Opponent Company

CARPMAELS & RANSFORD (SPECIALITIES) LLP

1 Southampton Row, GB

Representative

Carpmaels & Ransford LLP

Filing Date

2024-05-30

Opposition in Progress

Opponent Company

James Poole Limited

One Southampton Row, GB

Representative

Carpmaels & Ransford LLP

Filing Date

2024-05-30

Opposition in Progress

Opponent Company

ModernaTX, Inc.

325 Binney Street, US

Representative

Hoffmann Eitle

Filing Date

2026-05-20

Opposition in Progress

Legal Status & Event History

Official European Patent Office legal and procedural status changes recorded for this file.

74 Events Recorded

Date	Code	Event Description	Auth
2022-12-28	17P	REQUEST FOR EXAMINATION FILED	EP
2022-12-28	AC	DIVISIONAL APPLICATION: REFERENCE TO EARLIER APPLICATION	EP
2022-12-28	AK	DESIGNATED CONTRACTING STATES	EP
2022-11-25	PUAI	PUBLIC REFERENCE MADE UNDER ARTICLE 153(3) EPC TO A PUBLISHED INTERNATIONAL APPLICATION THAT HAS ENTERED THE EUROPEAN PHASE	EP
2022-11-25	STAA	INFORMATION ON THE STATUS OF AN EP PATENT APPLICATION OR GRANTED EP PATENT	EP
2023-03-02	REG	REFERENCE TO A NATIONAL CODE	EP
2023-03-03	STAA	INFORMATION ON THE STATUS OF AN EP PATENT APPLICATION OR GRANTED EP PATENT	EP
2023-04-05	17Q	FIRST EXAMINATION REPORT DESPATCHED	EP
2023-05-10	RAP3	PARTY DATA CHANGED (APPLICANT DATA CHANGED OR RIGHTS OF AN APPLICATION TRANSFERRED)	EP
2023-07-18	GRAP	DESPATCH OF COMMUNICATION OF INTENTION TO GRANT A PATENT	EP
2023-07-18	STAA	INFORMATION ON THE STATUS OF AN EP PATENT APPLICATION OR GRANTED EP PATENT	EP
2023-07-21	GRAS	GRANT FEE PAID	EP
2023-07-28	GRAA	(EXPECTED) GRANT	EP
2023-07-28	STAA	INFORMATION ON THE STATUS OF AN EP PATENT APPLICATION OR GRANTED EP PATENT	EP
2023-08-16	INTG	INTENTION TO GRANT ANNOUNCED	EP
2023-08-11	REG	REFERENCE TO A NATIONAL CODE	EP
2023-08-30	AC	DIVISIONAL APPLICATION: REFERENCE TO EARLIER APPLICATION	EP
2023-08-30	AK	DESIGNATED CONTRACTING STATES	EP
2023-08-30	REG	REFERENCE TO A NATIONAL CODE	EP
2023-08-31	REG	REFERENCE TO A NATIONAL CODE	EP
2023-09-20	P01	OPT-OUT OF THE COMPETENCE OF THE UNIFIED PATENT COURT (UPC) REGISTERED	EP
2023-09-21	REG	REFERENCE TO A NATIONAL CODE	EP
2023-10-02	REG	REFERENCE TO A NATIONAL CODE	EP
2023-10-09	REG	REFERENCE TO A NATIONAL CODE	EP
2023-10-17	REG	REFERENCE TO A NATIONAL CODE	EP
2023-10-25	REG	REFERENCE TO A NATIONAL CODE	EP
2023-10-10	REG	REFERENCE TO A NATIONAL CODE	EP
2023-09-27	REG	REFERENCE TO A NATIONAL CODE	EP
2023-12-27	REG	REFERENCE TO A NATIONAL CODE	EP
2024-01-18	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2024-01-23	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2024-01-31	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2024-03-01	PLBI	OPPOSITION FILED	EP
2024-03-04	REG	REFERENCE TO A NATIONAL CODE	EP
2024-02-27	REG	REFERENCE TO A NATIONAL CODE	EP
2023-06-19	REG	REFERENCE TO A NATIONAL CODE	EP
2024-03-15	REG	REFERENCE TO A NATIONAL CODE	EP
2024-04-03	26	OPPOSITION FILED	EP
2024-04-30	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2024-05-31	PLBI	OPPOSITION FILED	EP
2024-05-31	PLAB	OPPOSITION DATA, OPPONENT'S DATA OR THAT OF THE OPPONENT'S REPRESENTATIVE MODIFIED	EP
2024-06-07	PLBI	OPPOSITION FILED	EP
2024-06-07	PLAB	OPPOSITION DATA, OPPONENT'S DATA OR THAT OF THE OPPONENT'S REPRESENTATIVE MODIFIED	EP
2024-06-11	PLAX	NOTICE OF OPPOSITION AND REQUEST TO FILE OBSERVATION + TIME LIMIT SENT	EP
2024-07-03	26	OPPOSITION FILED	EP
2024-07-03	R26	OPPOSITION FILED (CORRECTED)	EP
2024-07-10	R26	OPPOSITION FILED (CORRECTED)	EP
2024-07-10	26	OPPOSITION FILED	EP
2024-07-31	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2024-09-03	PLAF	INFORMATION MODIFIED RELATED TO COMMUNICATION OF A NOTICE OF OPPOSITION AND REQUEST TO FILE OBSERVATIONS + TIME LIMIT	EP
2024-11-08	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2024-11-29	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2024-12-13	PLBB	REPLY OF PATENT PROPRIETOR TO NOTICE(S) OF OPPOSITION RECEIVED	EP
2024-12-16	REG	REFERENCE TO A NATIONAL CODE	EP
2025-01-08	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2025-01-16	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2025-01-31	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2025-07-23	PGFP	ANNUAL FEE PAID TO NATIONAL OFFICE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2025-09-12	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-02-06	PLBP	OPPOSITION WITHDRAWN	EP
2026-02-11	REG	REFERENCE TO A NATIONAL CODE	EP
2026-03-06	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-05-28	REG	REFERENCE TO A NATIONAL CODE	EP
2026-06-01	PG25	LAPSED IN A CONTRACTING STATE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-06-15	PGFP	ANNUAL FEE PAID TO NATIONAL OFFICE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-07-08	PGFP	ANNUAL FEE PAID TO NATIONAL OFFICE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-07-09	PGFP	ANNUAL FEE PAID TO NATIONAL OFFICE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-07-10	PGFP	ANNUAL FEE PAID TO NATIONAL OFFICE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-07-11	PGFP	ANNUAL FEE PAID TO NATIONAL OFFICE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-07-15	PGFP	ANNUAL FEE PAID TO NATIONAL OFFICE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-07-16	PGFP	ANNUAL FEE PAID TO NATIONAL OFFICE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-07-18	PGFP	ANNUAL FEE PAID TO NATIONAL OFFICE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-07-23	PGFP	ANNUAL FEE PAID TO NATIONAL OFFICE [ANNOUNCED VIA POSTGRANT INFORMATION FROM NATIONAL OFFICE TO EPO]	EP
2026-08-20	REG	REFERENCE TO A NATIONAL CODE	EP

Patent & Non-Patent Citations

Cited documents and prior art referenced in search and examination reports.

82 Citations

Cited Patent Documents

WO2008077592

Espacenet

WO2014140211

Espacenet

WO2014152966

Espacenet

CA2277165 1998-07-16

Espacenet

WO0107599 2001-02-01

Espacenet

US2002096474 2002-07-25

Espacenet

WO2014152027 2014-09-25

Espacenet

WO2014152031 2014-09-25

Espacenet

US6011148

Espacenet

US2007207493

Espacenet

WO2010027955

Espacenet

WO2012077080

Espacenet

WO9204970

Espacenet

WO2015133972

Espacenet

WO2013064911

Espacenet

WO2015164773

Espacenet

US2004076980

Espacenet

US8075780

Espacenet

WO2016193206

Espacenet

WO03082892

Espacenet

WO2004076695

Espacenet

WO2015200691

Espacenet

EP1533020

Espacenet

WO2008107462 2008-09-12

Espacenet

Non-Patent Scientific Literature (NPL)

- PASCOLO ET AL., METHODS MOL MED, vol. 127, 2006, pages 23 - 40

- FERNANDEZ ET AL., A BIOTECHNOLOGICA, vol. 12, pages 49 - 56

- RATHORE, AS ET AL., PREP BIOCHEM BIOTECHNOL, vol. 41, no. 4, 2011, pages 398 - 421

- A. AZARANIK.H. HECKER, NUCLEIC ACIDS RESEARCH, vol. 29, no. 2, pages e7

- SAMBROOKRUSSEL: "Molecular Cloning: A Laboratory Manual", vol. 3, 2000, COLD SPRING HARBOR LABORATORY

- FLORES ET AL., PLANT PHYSIOL., vol. 69, 1982, pages 701 - 706

- SAMBROOK ET AL.: "Molecular Cloning, a laboratory manual", 1989, COLD SPRING HARBOR LABORATORY PRESS

- NESTOLA PIERGIUSEPPE ET AL: "Evaluation of novel large cut-off ultrafiltration membranes for adenovirus serotype 5 (Ad5) concentration.", PLOS ONE, vol. 9, no. 12, E115802, 29 December 2014 (2014-12-29), pages 1 - 22, XP002760961, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0115802

- ALEX EON-DUVAL ET AL: "Removal of RNA impurities by tangential flow filtration in an RNase-free plasmid DNA purification process", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC, NEW YORK, vol. 316, no. 1, 1 May 2003 (2003-05-01), pages 66 - 73, XP002622156, ISSN: 0003-2697, [retrieved on 20030402], DOI: 10.1016/S0003-2697(03)00050-2

- ANONYMOUS: "Cross Flow Filtration Method Handbook", 1 January 2014 (2014-01-01), pages 1 - 82, XP055198384, Retrieved from the Internet <URL:https://www.gelifesciences.com/gehcls_images/GELS/Related Content/Files/1392028292867/litdoc29085076_20140313045908.pdf> [retrieved on 20150625]

- "A LABORATORY GUIDE TO RNA, Isolation, Analysis, and Synthesis, ", 1 January 1996, WILEY-LISS, article KRIEG PAUL A: "MATERIALS AND METHODS Materials for Production of Synthetic mRNA", pages: 143 - 145, XP093191508

- OLSON WAYNE P.: "Separations TECHNOLOGY, Pharmaceutical and Biotechnology Applications", 1 January 2015, CRC PRESS, article MICHAELIS, STEPHEN L. ET AL: "Tangential Flow Filtration", pages: 1 - 20, XP093191574

- ANONYMOUS: "Protein Concentration and Diafiltration by Tangential Flow Filtration", MILLIPORE, 1 January 2003 (2003-01-01), pages 1 - 24, XP093191710

- WILLIAMS N T: "Continuous Production of RNA using a Stir-cell Bioreactor", THESIS, 22 March 1995 (1995-03-22), pages 1 - 132, XP093136738

- ANONYMOUS: "Sartocon® 2Plus Stainless Steel Holder - Crossflow holder for Sartocon® cassettes", SARTORIUS, 24 September 2014 (2014-09-24), XP093191802

- NESTOLA PIERGIUSEPPE ET AL: "Evaluation of novel large cut-off ultrafiltration membranes for adenovirus serotype 5 (Ad5) concentration.", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 9, no. 12, 1 January 2014 (2014-01-01), US , pages e115802, XP002760961, ISSN: 1932-6203

- ALEX EON-DUVAL, ROBERT H. MACDUFF, CAROL A. FISHER, MARK J. HARRIS, CHRIS BROOK: "Removal of RNA impurities by tangential flow filtration in an RNase-free plasmid DNA purification process", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 316, no. 1, 1 May 2003 (2003-05-01), Amsterdam, NL , pages 66 - 73, XP002622156, ISSN: 0003-2697, DOI: 10.1016/S0003-2697(03)00050-2

- "Cross Flow Filtration Method Handbook", 1 January 2014 (2014-01-01), XP055198384, Retrieved from the Internet <URL:https://www.gelifesciences.com/gehcls_images/GELS/Related Content/Files/1392028292867/litdoc29085076_20140313045908.pdf> [retrieved on 20150625]

- ANONYMOUS: "Filtration Selection Guide - Choosing the Right Filter for Your Application", LENNTECH, 1 December 2004 (2004-12-01), XP093191814, Retrieved from the Internet <URL:https://www.lenntech.com/Data-sheets/Millipore-Filtration-Selection-Guide-L.pdf>

- ANONYMOUS: "Products and Solutions for the Biopharmaceutical Industry", SARTORIUS STEDIM BIOTECH DOCUMENT, SARTORIUS STEDIM BIOTECH GMBH, DE, 1 January 2010 (2010-01-01), DE, pages 1 - 642, XP009556313

- ANONYMOUS: "Centricon - Centrifugal filter devices - user guide", 1 March 2005 (2005-03-01), pages 1 - 23, XP002600140, Retrieved from the Internet <URL:http://kirschner.med.harvard.edu/files/protocols/Millipore_Centricons.pdf> [retrieved on 20100909]

- HUI F. LIU, MA JUNFEN, WINTER CHARLES, BAYER ROBERT: "Recovery and purification process development for monoclonal antibody production", MABS, vol. 2, no. 5, 1 September 2010 (2010-09-01), pages 480 - 499, XP055027612, ISSN: 19420862, DOI: 10.4161/mabs.2.5.12645

- RIVERA ESTEBAN, DOMINGUEZ CARLOS A, ESCOBAR CARLOS, WEIDNER JAMES: "Improving Tangential Flow Filtration Yield", BIOPHARM INTERNATIONAL, ADVANSTAR COMMUNICATIONS, DULUTH, MN, US, vol. 21, no. 7, 1 July 2008 (2008-07-01), US , pages 42, XP093191069, ISSN: 1542-166X

- "RNA : Methods and Protocols : Methods and Protocols", vol. 703, 1 January 2011, HUMANA PRESS, Totowa, NJ, ISBN: 978-1-59745-248-9, ISSN: 1064-3745, article BECKERT BERTRAND, MASQUIDA BENOÎT: "Synthesis of RNA by In Vitro Transcription : Methods and Protocols", pages: 29 - 41, XP093153524, DOI: 10.1007/978-1-59745-248-9_3

- ANONYMOUS: "The Partnership of the Minimate TM TFF Capsule with Liquid Chromatography Systems Facilitates Lab-scale Purifications and Process Development Through In-Line Monitoring", PALL LIFE SCIENCES; SCIENTIFIC & TECHNICAL REPORT PN33339, 1 April 2010 (2010-04-01), XP093191073

- ANONYMOUS: "Pall Omega™ Membrane Cassettes - Validation Guide USTR 2143 Rev B", PALL LIFE SCIENCES, 1 January 2007 (2007-01-01), XP093191076

- A. S. RATHORE, A. SHIRKE: "RECENT DEVELOPMENTS IN MEMBRANE-BASED SEPARATIONS IN BIOTECHNOLOGY PROCESSES: REVIEW", PREPARATIVE BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 41, no. 4, 1 October 2011 (2011-10-01), pages 398 - 421, XP055153231, ISSN: 10826068, DOI: 10.1080/10826068.2011.613976

- BECKWITH MICHELLE, BACKER ANDREW, ROBERTSON ANDREW, PHILLIPS WHITNEY, JIMENEZ MALENA, BALDWIN BRITTANY, VAGELL MICHAEL: "The Role Of Ultrafiltration Membranes In The Recovery Of DNA With Centrifugal Concentrators", INTERNATIONAL SYMPOSIUM ON HUMAN IDENTIFICATION, 1 October 2014 (2014-10-01) - 14 October 2014 (2014-10-14), XP093191078

- ALONSO SARA V: "Implementation of a New Membrane and the Re-use in a Tangential Flow Filtration System", MANUFACTURING ENGINEERING, 1 January 2014 (2014-01-01), XP093191083

- THOMAS PAUL: "Tangential Flow Filtration Explores New Niches", PHARMA MANUFACTURING, 19 August 2008 (2008-08-19), XP093191084, Retrieved from the Internet <URL:https://www.pharmamanufacturing.com/home/article/11362204/pharmaceutical-flow-filtration-tangential-flow-filtration-explores-new-niches-pharmaceutical-manufacturing>

- YOUNG CLARE: "Pall Corporation introduces T-series cassettes for TFF applications News", FILTRATION + SEPARATION, 29 April 2010 (2010-04-29), XP093191087, Retrieved from the Internet <URL:https://www.filtsep.com/content/news/pall-corporation-introduces-t-series-cassettes-for-tff-applications/>

- JAGAN NATHAN S, SHIVANANDAPPA K C, SUNDRAN B K N, VENKATARAMANA K R, MANI: "Filtration Technique in Vaccine Manufacturing", TOOLS & TECHNIQUES, 1 August 2008 (2008-08-01), pages 37, XP093191090

- "DNA Vaccines", vol. 127, 1 January 2006, HUMANA PRESS, Totowa, NJ, ISBN: 9781588294845, ISSN: 1543-1894, article MURPHY JASON C., WINTERS MICHAEL A., SAGAR SANGEETHA L.: "Chapter 24: Large-Scale, Nonchromatographic Purification of Plasmid DNA", pages: 351 - 362, XP009556210, DOI: 10.1385/1-59745-168-1:351

- SCHWARTZ, LARRY; SEELEY, KEVIN: "Introduction to Tangential Flow Filtration for Laboratory and Process Development Applications", SCIENTIFIC & TECHNICAL REPORT, PALL LIFE SCIENCES, ANN ARBOR, US, Ann Arbor, US, pages 1 - 12, XP055698772, Retrieved from the Internet <URL:https://www.pall.com/content/dam/pall/laboratory/literature-library/non-gated/Molecular_Tech_Report_Minimate_Intro_to_TFF.pdf> [retrieved on 20200527]

- JANSON, JAN-CHRISTER: "Protein Purification", 22 March 2011, JOHN WILEY & SONS, INC. , Hoboken, NJ, USA , ISBN: 978-0-471-74661-4, article JOACHIM K. WALTER, ZUWEI JIN, MAIK W. JORNITZ, UWE GOTTSCHALK: "Chapter 12: Membrane Separations", pages: 281 - 317, XP009556204

- "Nucleic acids / Series title: Methods in Enzymology", vol. 12, 1 January 1968, ELSEVIER SCIENCE & TECHNOLOGY, ISBN: 9780121818562, ISSN: 0076-6879, article BOEDTKER HELGA: "[129] Molecular weight and conformation of RNA", pages: 429 - 458, XP009556211, DOI: 10.1016/0076-6879(67)12154-X

- "Membranes for the Life Sciences", 17 October 2007, WILEY, article ALLEGREZZA ANTHONY, IRELAND TODD, KOOLS WILLEM, PHILLIPS MICHAEL, RAGHUNATH BALA, WILKINS RANDY, XENOPOULOS ALEX: "Membranes in the Biopharmaceutical Industry", pages: 91 - 153, XP093191013, DOI: 10.1002/9783527631360.ch4 * p. 91-153 *

- "Current Protocols in Nucleic Acid Chemistry", 1 January 2011, JOHN WILEY & SONS, INC. , Hoboken, NJ, USA , ISBN: 9780471142706, article STEFANIE HOFFMANN, JOHANNES HOOS, SVEN KLUSSMANN, STEFAN VONHOFF: "RNA Aptamers and Spiegelmers: Synthesis, Purification, and Post-Synthetic PEG Conjugation", XP055058007, DOI: 10.1002/0471142700.nc0446s46 * 4.46.1 to 4.46.30 *

- JULIE L. BRUNELLE ET AL.: "In Vitro Transcription from Plasmid or PCR-amplified DNA", METHODS IN ENZYMOLOGY, vol. 530, 2013, XP009190475 * 101-114 *

- "Separations Technology, Pharmaceutical and Biotechnology Applications", 1 January 1995, TAYLOR & FRANCIS LTD, ISBN: 9780935184723, article STEPHEN L. MICHAELS, CHRIS ANTONIOU, VINAY GOEL, PAULA KEATING, RALF KURIYEL, ALAN S. MICHAELS, STEVEN R. PEARL, GASTON DE LOS REY: "3 Tangential Flow Filtration", pages: 57 - 194, XP009556274 * 57-194 *

- KIM L. HOSSNER ET AL.: "Improved recovery of insulin-like growth factors (IGFs) from bovine colostrum using alkaline diafiltration", BIOTECHNOL. APPL. BIOCHEM. , vol. 32, 2000, XP008085428, DOI: 10.1042/BA20000056 * 161-166 *

- RAYMOND H COLTON, INA PAHL, LIANA E OTTAVIANO, TRAVIS BODEUTSCH, FRANK MEYEROLTMANNS: "Study of Protein Adsorption Effects on Crossflow Filtration Using BSA and Milk Protein", PDA JOURNAL OF PHARMACEUTICAL SCIENCE AND TECHNOLOGY, PARENTERAL DRUG ASSOCIATION, US, vol. 56, no. 1, 1 January 2002 (2002-01-01), US , pages 20 - 30, XP009556243, ISSN: 1079-7440 * 20-30 *

- ANONYMOUS: "Pellicon® 2 vs. Hollow Fiber Filter Cartridge - Application Note AN1020EN00", MILLIPORE APPLICATION NOTE, MILLIPORE, 1 May 2001 (2001-05-01), pages 1 - 8, XP009558957

- ANONYMOUS: "Sartocon® Hydrosart® Cassette 3021446830E-BSW", SARTORIUS, 11 May 2015 (2015-05-11), XP093191032

- ANONYMOUS: "UltracelTM PLC Membranes - Data Sheet", MILLIPORE, 1 January 2002 (2002-01-01), XP093191035

- ANONYMOUS: "Labscale ™ Tangential Flow Filtration System - User Guide", MILLIPORE, 1 April 2011 (2011-04-01), XP093191038, Retrieved from the Internet <URL:https://labtorg.kz/downloads/Millipore/Labscale-Tangential-Flow-Filtration-System-User-Guide.pdf>

- D63 - Notification of the recording of a change for PCT/EP2015/062002 mailed on 26 October 2015

- D64 - PCT request for PCT/EP2016/062152

- D65 - Communication of the EPO re 16729807.4 dated 7 February 2020

- D66 - Request for grant of a European patent (EP20166485.1)

- D67 - Request for grant of a European patent (EP22190069.9)

- J. KOOLMAN, K.H. ROEHM: "Color Atlas of Biochemistry, 2nd Edition", 1 January 2005, THIEME, ISBN: 1-58890-247-1, article KOOLMAN, JAN; RÖHM, KLAUS-HEINRICH: "Basics and Biomolecules", pages: 1 - 87, XP009559384

- ANONYMOUS: "KrosFlo Research IIi TFF System", SPECTRUMLABS.COM, vol. 67, no. 24, pages 21717 - 21728, XP093249026, Retrieved from the Internet <URL:https://cdn.thomasnet.com/ccp/01300199/133476.pdf> DOI: 10.1021/acs.jmedchem.4c01725

- SOPHIA T. MUNDLE, HECTOR HERNANDEZ, JOHN HAMBERGER, JOHN CATALAN, CHANGHONG ZHOU, SVETLANA STEGALKINA, ANDREA TIFFANY, HARRY KLEAN: "High-Purity Preparation of HSV-2 Vaccine Candidate ACAM529 Is Immunogenic and Efficacious In Vivo", PLOS ONE, vol. 8, no. 2, pages e57224, XP055180742, DOI: 10.1371/journal.pone.0057224

- D75 - UPC Case Overview

- D76 - Excerpt from UPC CMS showing 13 May 2016 as the effective date of service on the Intervener

- LABISCH JENNIFER J., EVANGELOPOULOU MARIA, SCHLEUSS TOBIAS, PICKL ANDREAS: "Investigating Ultrafiltration Membranes and Operation Modes for Improved Lentiviral Vector Processing", ENGINEERING IN LIFE SCIENCES, WILEY, WEINHEIM, DE, vol. 25, no. 1, 1 January 2025 (2025-01-01), DE , XP093401048, ISSN: 1618-0240, DOI: 10.1002/elsc.202400057

Worldwide Patent Family

Related applications and patents in the same INPADOC worldwide family.

40 Family Members

Country	Publication Number	Kind	Publication Date	Action
EP	EP4108769A1	A1	2022-12-28	Espacenet
EP	EP4108769B1	B1	2023-08-30	Espacenet
CN	CN107873055A	A	2018-04-03	Espacenet
CN	CN107873055B	B	2021-09-17	Espacenet
CN	CN113897350A	A	2022-01-07	Espacenet
CN	CN113897350B	B	2026-09-01	Espacenet
DE	DE202016009003U1	U1	2021-05-28	Espacenet
DE	DE22190069T1	T1	2023-03-02	Espacenet
DK	DK3303583T3	T3	2020-07-13	Espacenet
DK	DK4108769T3	T3	2023-10-09	Espacenet
EP	EP3303583A1	A1	2018-04-11	Espacenet
EP	EP3303583B1	B1	2020-04-01	Espacenet
EP	EP3744843A1	A1	2020-12-02	Espacenet
EP	EP4660314A2	A2	2025-12-10	Espacenet
EP	EP4660314A3	A3	2026-03-11	Espacenet
EP	EP4660315A2	A2	2025-12-10	Espacenet
EP	EP4660315A3	A3	2026-03-18	Espacenet
ES	ES2798271T3	T3	2020-12-10	Espacenet
ES	ES2960429T3	T3	2024-03-04	Espacenet
ES	ES3067295T1	T1	2026-05-21	Espacenet
ES	ES3067304T1	T1	2026-05-21	Espacenet
PL	PL3303583T3	T3	2020-10-19	Espacenet
PL	PL4108769T3	T3	2024-02-05	Espacenet
PT	PT3303583T	T	2020-07-07	Espacenet
PT	PT4108769T	T	2023-10-10	Espacenet
US	US2018298372A1	A1	2018-10-18	Espacenet
US	US10760070B2	B2	2020-09-01	Espacenet
US	US2021040473A1	A1	2021-02-11	Espacenet
US	US11274293B2	B2	2022-03-15	Espacenet
US	US2022325273A1	A1	2022-10-13	Espacenet
US	US11667910B2	B2	2023-06-06	Espacenet
US	US2023151352A1	A1	2023-05-18	Espacenet
US	US11760992B2	B2	2023-09-19	Espacenet
US	US2023287388A1	A1	2023-09-14	Espacenet
US	US11834651B2	B2	2023-12-05	Espacenet
US	US20240247249A1	A1	2024-07-25	Espacenet
US	US12221605B2	B2	2025-02-11	Espacenet
US	US20250129359A1	A1	2025-04-24	Espacenet
US	US20260043019A1	A1	2026-02-12	Espacenet
WO	WO2016193206A1	A1	2016-12-08	Espacenet

Official File Inspection EPC Art. 128 Public Dossier

Complete European Patent Register Dossier (148 Total Documents)

The full inspection file for Application EP22190069 contains 148 total documents, including all notices of opposition, proprietor counter-statements, examination communications, third-party observations, and procedural forms. Below are all major recorded procedural filings and publication specifications.

Open 148-Doc Register Dossier

Recorded Filings & Patent Specifications

Official publication specifications, opposition filings, proprietor replies, and procedural submissions.

64 Filings Listed

European Patent Specification - Granted Patent (B1)

Publication Specification

Date: 2023-08-30 Procedure: Grant EP4108769B1

View / Download PDF

Official European Patent Register Full Dossier

Publication Specification

Date: 2023-08-30 Procedure: All Proceedings (Examination & Opposition) EP22190069

View / Download PDF

European Patent Application with Search Report (A1)

Publication Specification

Date: 2022-12-28 Procedure: Publication EP4108769A1

View / Download PDF

Oral proceedings

Procedural Step

Date: 2026-12-14 Procedure: Examination ORAL

Inspect on Register

New entry: Date of oral proceedings

Dossier Filing

Date: 2026-07-02 Procedure: Examination EPIDOSNORA1O

Inspect on Register

Deletion: Date of oral proceedings

Dossier Filing

Date: 2026-06-16 Procedure: Examination EPIDOSDORA1O

Inspect on Register

Deletion: Date of cancellation oral proceedings

Dossier Filing

Date: 2026-06-16 Procedure: Examination EPIDOSDORA6O

Inspect on Register

Oral proceedings

Procedural Step

Date: 2026-06-09 Procedure: Examination ORAL

Inspect on Register

Lapse of the patent in a contracting state

Dossier Filing

Date: 2026-06-05 Procedure: Examination 0009250

Inspect on Register

New entry: Date of cancellation oral proceedings

Dossier Filing

Date: 2026-05-23 Procedure: Examination EPIDOSNORA6O

Inspect on Register

Intervention of assumed infringer

Dossier Filing

Date: 2026-05-22 Procedure: Examination 0009265

Inspect on Register

Oral proceedings

Procedural Step

Date: 2026-03-23 Procedure: Examination ORAL

Inspect on Register

A METHOD FOR PRODUCING AND PURIFYING RNA, COMPRISING AT LEAST ONE STEP OF TANGENTIAL FLOW FILTRATION

Patent Abstract / Summary

IPC Classifications

Priority Claims

Designated Contracting States (38 EPC States)

Parties & Representation

Applicants

Inventors (7)

Representatives / Agents

Key Procedural Dates

Patent Oppositions

COHAUSZ & FLORACK Patent- und Rechtsanwälte Partnerschaftsgesellschaft mbB

Höpfner, Sebastian

Pfizer Inc.

CARPMAELS & RANSFORD (SPECIALITIES) LLP

James Poole Limited

ModernaTX, Inc.

Legal Status & Event History

Patent & Non-Patent Citations

Cited Patent Documents

Non-Patent Scientific Literature (NPL)

Worldwide Patent Family

Complete European Patent Register Dossier (148 Total Documents)

Recorded Filings & Patent Specifications

European Patent Specification - Granted Patent (B1)

Official European Patent Register Full Dossier

European Patent Application with Search Report (A1)

Oral proceedings

New entry: Date of oral proceedings

Deletion: Date of oral proceedings

Deletion: Date of cancellation oral proceedings

Oral proceedings

Lapse of the patent in a contracting state

New entry: Date of cancellation oral proceedings

Intervention of assumed infringer

Oral proceedings

Lapse of the patent in a contracting state

Opposition withdrawn

New entry: Date of oral proceedings

Deletion: Date of oral proceedings

Deletion: Date of cancellation oral proceedings

Lapse of the patent in a contracting state

New entry: Date of cancellation oral proceedings

New entry: Date of oral proceedings

Lapse of the patent in a contracting state

Lapse of the patent in a contracting state

Change - representative

New entry: Reply of patent proprietor to notice(s) of opposition

Lapse of the patent in a contracting state

Change: Communication of a notice of opposition and request to file observations + time limit

Lapse of the patent in a contracting state

Invitation to proprietor to file observations on the notice of opposition

New entry: Communication of a notice of opposition and request to file observations + time limit

Opposition filed

Change - opposition data/opponent's data or that of the opponent's representative

Lapse of the patent in a contracting state

Opposition filed

Change - opposition data/opponent's data or that of the opponent's representative

Lapse of the patent in a contracting state

Lapse of the patent in a contracting state

Opposition filed

Lapse of the patent in a contracting state

Lapse of the patent in a contracting state

Lapse of the patent in a contracting state

Lapse of the patent in a contracting state

Lapse of the patent in a contracting state

(Expected) grant

New entry: Payment of fee for grant

New entry: Payment of fee for publishing/printing

New entry: Receipt of the translation of the claim(s)

Intention to grant the patent

New entry: Communication of intention to grant a patent

New entry: Renewal fee paid

Renewal fee payment

Change - applicant

New entry: Reply to examination report

New entry: Despatch of examination report + time limit

Communication from the examining division

First examination report

Publication in section I.1 EP Bulletin